SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Kikuti,M; Langoni,H; Nobrega,DN; Corrêa,APFL; Ullmann,LS.

Leptospirosis is a globally distributed emerging zoonosis. Dogs are commonly affected and although other serovars can cause canine leptospirosis, Leptospira interrogans serovar Canicola is primary found in these animals. A retrospective study was conducted using a database of 1195 dogs tested for Leptospira infection from 2003 to 2010 at the Laboratory of Zoonosis Diagnosis at the Veterinary Hospital of São Paulo State University (UNESP) in Botucatu, São Paulo state, Brazil. The seroprevalence of infected dogs was 20.08% (240/1195), and the most prevalent serovars were Canicola (6.7%), Copenhageni (5.0%), Icterohaemorrhagiae (2.9%), Autumnalis (2.9%), Pyrogenes (2.8%), Pomona (2.0%), Hardjo (2.0%), Australis (1.8%), Bratislava (1.6%), Cynopteri (1.4%),...

Leptospira spp. infection in sheep herds in southeast Brazil

Palavras-chave: Leptospira spp.; Dog; Serology; Epidemiology.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100016

Barbante,Priscila; Shimabukuro,Fabio H; Langoni,Helio; Richini-Pereira,Virgínia B; Lucheis,Simone B.

Background With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys...

Serological profile of anti-Toxoplasma gondii antibodies in apparently healthy dogs of the city of Botucatu, São Paulo State, Brazil

Palavras-chave: Leptospirosis; Ovine; Serology; Culture; PCR.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200325

Anti-leptospirose agglutinins in equine sera, from São Paulo, Goias, and Mato Grosso do Sul, Brazil, 1996-2001

Langoni,H.; Modolo,J. R.; Pezerico,S. B.; Silva,R. C.; Castro,A. P. B.; Silva,A. V. da; Padovani,C. R..

Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, a coccidian protozoan of worldwide distribution. The seroprevalence in canine population can be an alternative for measuring T. gondii urban spreading. A total of 780 blood samples from dogs were collected, during the yearly anti-rabies campaign, carried out by the Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Husbandry (FMVZ), São Paulo State University, UNESP, together with the county health authorities, in August 1999. Using Indirect Fluorescent Antibody Test (IFAT) for detecting antibodies anti-T. gondii in the sera samples, we observed that 258 dogs (33.1%) were positive. The associations between the serological results and the epidemiological...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Toxoplasma gondii; Dog; Serology.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100012

Langoni,H.; Da Silva,A.V.; Pezerico,S.B.; De Lima,V.Y..

Equine leptospirosis can present a non-symptomatic form, an acute clinical form, or even develop chronically, causing reproductive alterations, such as abortion and recurrent uveitis. Since the prevalence of leptospirosis in several countries and regions is widely reported, the objective of this study was to verify the prevailing equine leptospirosis in different regions of Brazil. Sera from 1402 blood samples from horses of different age, sex, breed, and purpose were examined. These samples came from southeastern and central west states of Brazil. The method utilized was the Microscopic Agglutination Test (MAT), with 12 different Leptospira serovars. From the sera tested, 754 (54%) were positive for one (385) or more (372) serovars. These results were...

American visceral Leishmaniasis: a case report

Palavras-chave: Leptospira; Horse; Serology.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300003

Langoni,H.; Lucheis,S. B.; Da Silva,R. C.; Castro,A. P. B.; Paes,A. C..

Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT) demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of...

Comparative serology of the marine fish pathogen Vibrio anguillarum

Palavras-chave: Leishmania; Zoonosis; Dog; Diagnosis; Hemoculture; Serology; Polymerase chain reaction (PCR).

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300012

OMA

Grisez, L.; Ollevier, F.P..

The different serotyping systems, based on thermostable O antigens, reported for Vibrio anguillarum and V. ordalii were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (LPS), using polyclonal rabbit antisera. The results demonstrate that 16 different serotypes within V. anguillarum (designated 01 to 016) can be distinguished. Each of these serotypes is characterized by a distinct polysaccharide banding pattern, as revealed by silver-stained gels of purified LPS. The comparative analysis allowed a complete alignment of the different stereotypes for the...

Simple and multiple resistances to viruses in cowpea genotypes

Palavras-chave: Pathogens; Serology; Vibrio anguillarum.

Ano: 1995

URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2970

PAB

Lima,José Albersio Araujo; Silva,Ana Kelly Firmino da; Aragão,Maria do Livramento; Ferreira,Nádia Rutielly de Araújo; Teófilo,Elizita Maria.

The objective of this work was to identify new sources of simple and multiple resistances to Cowpea severe mosaic virus (CPSMV), Cowpea aphid-borne mosaic virus (CABMV) and Cucumber mosaic virus (CMV) isolates in cowpea (Vigna unguiculata). Thirty-three genotypes from the germplasm bank of Universidade Federal do Ceará were tested as to their resistance to four CPSMV isolates, two CABMV isolates and one CMV isolate. Twenty-five days after the first virus inoculations, all inoculated plants, including the asymptomatic ones, were tested by serology. Genotypes were classified as: immune, plants without symptoms and negative serology; resistant, plants with mild mosaic and positive serology; susceptible, plants with mosaic and positive serology; and highly...

Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa

Palavras-chave: Cowpea aphid-borne mosaic virus; Cowpea severe mosaic virus; Cucumber mosaic virus; Vigna unguiculata; Serology; Virus control.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001100003

Oliveira,GH; Berchieri Jr,A; Montassier,HJ.

This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.

Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa

Palavras-chave: Chicken; ELISA; Enzyme; Salmonella sp; Serology.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008

Oliveira,GH de<A NAME="nt01a"></A>; Berchieri Júnior,Â; Montassier,HJ; Fernandes,AC.

This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.

Serosurvey of selected avian pathogens in brazilian commercial Rheas (Rhea americana) and Ostriches (Struthio camelus)

Palavras-chave: Chickens; Conjugate; ELISA; Salmonella sp; Serology.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007

Freitas Neto,OC de; Carrasco,AOT; Raso,TF; Sousa,RLM; Berchieri Júnior,A; Pinto,AA.

Ratite farming of has expanded worldwide. Due to the intensive farming methods used by ratite producers, preventive medicine practices should be established. In this context, the surveillance and control of some avian pathogens are essential for the success of the ratite industry; however, little is known on the health status of ratites in Brazil. Therefore, the prevalence of antibodies against Newcastle Disease virus, Chlamydophila psittaci, Mycoplasma gallisepticum, Mycoplasma synoviae, and Salmonella Pullorum were evaluated in 100 serum samples collected from commercial ostriches and in 80 serum samples from commercial rheas reared in Brazil. All sampled animals were clinically healthy. The results showed that all ostriches and rheas were serologically...

Serological Diagnosis of Influenza A Subtype H1 on Family Poultry of Belo Horizonte, Minas Gerais and Santa Maria, Rio Grande Do Sul, in Brazil.

Palavras-chave: Chlamydophila psittaci; Mycoplasma spp.; Newcastle Disease virus; Ostrich; Rhea; Salmonella spp.; Serology.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000400010

Castro Filho,RPL; Torres,ACD; Marin,SY; Diniz,SA; Matos,JCC; Mureb,EN; Resende,M; Martins,NRS.

ABSTRACT Serum samples (n=687) from Gallus gallus domesticus were collected for the investigation of antibodies to avian influenza virus (AIV-A) in the family poultry of the surrounding counties of Santa Maria/RS and the metropolitan region of Belo Horizonte/MG, totaling twenty different counties. Additional samples of seventeen (n=17) free-flying ducks (C. moschata pure or hybrid with Anas platyrhynchos) were collected in Belo Horizonte. The chosen tests for the survey were performed as described by the World Organization for Animal Health (OIE), including agar gel immunodiffusion (AGID) for antibodies to AIV-A nucleoprotein (N) and haemagglutination- inhibition (HI) for antibodies to subtype H1. Out of the 704 serum tests performed by AGID, eight (8/704)...

Infectious bursal disease virus: case report and experimental studies in vaccinated and unvaccinated SPF chickens and commercial broiler chicks

Palavras-chave: Anas platyrhynchos; Avian influenza; Brazil; Cairina moschata; Family poultry; Gallus gallus domesticus; Serology.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400811

Scanavini Neto,H; Ito,NMK; Miyaji,CI; Lima,E de A; Okabayashi,S; Corrêa,ARA; Eleutério,GC; Zuanaze,MA.

IBDV Gm 11 (Simbios eleven-molecular group) has been detected since 1997 in many farms of commercial broilers and layers causing high mortality (2 to 15%) and severe macro and microscopic damage in cloacal bursae, spleen, thymus, kidney and liver. Five serial passages of 2050/97-Gm 11 IBDV sample by CAM route in SPF chicken's embryonated eggs did not elicit increased embryo mortality. High mortality (100%) of 21 day-old SPF leghorn chickens and severe bursal and splenic lesions were seen from 24 up to 48 hours after eye-drop inoculation of 2050/97 strain (50 mL of 10-2 dilution of 10% bursae homogenate). Mortality was not detected when vaccinated SPF and broiler chickens were inoculated. One dead bird was found among ten challenged unvaccinated broilers....

Serologic response of SPF chickens to live vaccines and other strains of Mycoplasma gallisepticum

Palavras-chave: Challenge; Histopathology; IBD; Pathogenicity; Serology.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100006

Nascimento,ER do; Polo,P de A; Pereira,VL de A; Barreto,ML; Nascimento,M da GF do; Zuanaze,MAF; Corrêa,ARA; Silva,R de CF.

False positive serologic reactions and difficulties in the diagnosis of Mycoplasma gallisepticum (MG) in chickens have increased lately as a result of infection by low virulent MG strains and the use of live MG vaccines in poultry. The objective of this study was to evaluate the serologic responses of SPF chickens exposed to the three commercially available live MG vaccines, and one low virulent MG strain (MG-70), contributing to the diagnosis and monitoring of MG infection in birds. Six groups of SPF chickens were used. The control group was not infected nor challenged; one group was infected with the low virulent strain MG-70 (MG-70); three groups were immunized and named after the MG vaccine used, i.e., MG-6/85, MG-ts11, and MG-F; and finally one group...

Test profiles of broiler breeder flocks housed in farms with endemic Mycoplasma synoviae infection

Palavras-chave: Mycoplasma; Poultry; Serology; Vaccination.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100007

Fiorentin,L; Mores,MAZ; Trevisol,IM; Antunes,SC; Costa,JLA; Soncini,RA; Vieira,ND.

There is a need for a better understanding of the epidemiology of Mycoplasma synoviae (MS) infection in broiler breeders in Brazil. Many features of the infection remain unrecognizable, because there are no clinical signs of the disease. A detailed testing was performed at each 6 to 8 weeks in three MS-free flocks introduced in farms with endemic MS infection for a follow-up epidemiological study. Every flock was monitored by polymerase chain reaction (PCR), by serum plate agglutination (SPA) and hemagglutination inhibition (HI) for serology studies, and isolation of mycoplasmas from tracheal swabs. PCR was found to be the most sensitive test, detecting early MS infection. Serology was positive in less than 50% of the sera and MS was isolated only between...

Elisa evaluation of the levels of antibodies against Infectious Bronchitis Virus in laying hens using egg yolk as substrate

Palavras-chave: Broiler breeders; Mycoplasma synoviae; PCR; Serology; Survey; Transmission.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100005

Rauber,RH; Flôres,ML; Pereira,CE; Grigulo,M.

The present work was carried out to compare Infectious Bronchitis Virus (IBV) antibody titers in serum and egg yolk samples from laying hens. Sixty paired blood and egg samples were collected from laying hens of two farms. Serum samples were frozen, while egg yolk samples were diluted (1:500) before freezing. Serum and yolk samples were tested for the presence of IBV antibodies by indirect ELISA (commercial kit) and titers were compared by a correlation test (alpha=0.05). There was a high correlation (r=0.62) between the two kinds of samples, which means that titers of IBV antibodies in the egg yolk and in serum samples are quite the same. Considering that blood collection causes deep stress that leads to economic losses, and since eggs are collected daily...